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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    WB analysis of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66849-1-Ig (CPLX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66849-1-Ig (CPLX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 66849-1-Ig (same clone as 66849-1-PBS)

    FC experiment of SH-SY5Y using 66849-1-Ig (same clone as 66849-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CPLX2 Monoclonal antibody (66849-1-Ig, Clone:1B2A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

CPLX2 Monoclonal antibody, PBS Only

CPLX2 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 66849-1-PBS

产品说明书

CloneNo. 1B2A11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

CPX 2, CPLX 2, Complexin-2, 921L, 921 L

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66849-1-PBS targets CPLX2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CPLX2 fusion protein Ag27949 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 complexin 2
别名 CPX 2, CPLX 2, Complexin-2, 921L, 921 L
计算分子量 134 aa, 15 kDa
观测分子量 18-20 kDa
GenBank蛋白编号BC093706
基因名称 CPLX2
Gene ID (NCBI) 10814
RRIDAB_2882189
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ6PUV4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.