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CPLX2 Monoclonal antibody, PBS Only

CPLX2 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2A11

Cat no : 66849-1-PBS

Print datasheet

Synonyms

CPX 2, CPLX 2, Complexin-2, 921L, 921 L

验证数据展示

  • WB analysis of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    WB analysis of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66849-1-Ig (CPLX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66849-1-Ig (CPLX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 66849-1-Ig (same clone as 66849-1-PBS)

    FC experiment of SH-SY5Y using 66849-1-Ig (same clone as 66849-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CPLX2 Monoclonal antibody (66849-1-Ig, Clone:1B2A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

产品信息

66849-1-PBS targets CPLX2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CPLX2 fusion protein Ag27949 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complexin 2
Synonyms CPX 2, CPLX 2, Complexin-2, 921L, 921 L
Calculated Molecular Weight 134 aa, 15 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC093706
Gene Symbol CPLX2
Gene ID (NCBI) 10814
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6PUV4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.