CoraLite®594-conjugated CPLX2 Monoclonal antibody
CPLX2 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1B2A11
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | SH-SY5Y cells |
For other applications, we recommend the unconjugated version of this antibody, 66849-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66849 targets CPLX2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | CPLX2 fusion protein Ag27949 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | complexin 2 |
Synonyms | CPX 2, CPLX 2, Complexin-2, 921L, 921 L |
Calculated Molecular Weight | 134 aa, 15 kDa |
Observed Molecular Weight | 18-20 kDa |
GenBank Accession Number | BC093706 |
Gene Symbol | CPLX2 |
Gene ID (NCBI) | 10814 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q6PUV4 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.
实验方案
Product Specific Protocols | |
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FC protocol for CL594 CPLX2 antibody CL594-66849 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |