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- KD/KO Validated
CPNE1 Polyclonal antibody
CPNE1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293T cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CPNE1 Ready-To-Use IHC Kit. CPNE1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
10126-2-AP targets CPNE1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | CPNE1 fusion protein Ag0139 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | copine I |
| Synonyms | Copine 1, copine I, COPN1, CPN1, CPNE1 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC001142 |
| Gene Symbol | CPNE1 |
| Gene ID (NCBI) | 8904 |
| RRID | AB_2084202 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99829 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CPNE1 antibody 10126-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Carcinogenesis Copine 1 predicts poor clinical outcomes by promoting M2 macrophage activation in ovarian cancer | ||
MedComm (2020) Hsa-miR-335 enhances cell migration and invasion in lung adenocarcinoma through targeting Copine-1. | ||
Front Biosci (Landmark Ed) Silencing of CPNE1-TRAF2 Axis Restrains the Development of Pancreatic Cancer
| ||
Cell Death Discov Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p |
