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验证数据展示

  • FC experiment of HepG2 using 82952-2-RR

    FC experiment of HepG2 using 82952-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CPO Recombinant antibody (82952-2-RR, Clone:230153F4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HeLa using 82952-2-RR

    WB analysis of HeLa using 82952-2-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 82952-2-RR (CPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CPO Recombinant antibody

CPO Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82952-2-RR

Print datasheet

CloneNo. 230153F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

EC:3.4.17.-, Carboxypeptidase O, 230153F4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82952-2-RR targets CPO in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CPO fusion protein Ag24059 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name carboxypeptidase O
Synonyms EC:3.4.17.-, Carboxypeptidase O, 230153F4
Calculated Molecular Weight 374 aa, 43 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC112078
Gene Symbol CPO
Gene ID (NCBI) 130749
RRIDAB_3670695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IVL8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CPO antibody 82952-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols