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验证数据展示

  • IF Staining of HepG2 using CL488-18703

    IF Staining of HepG2 using CL488-18703

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CPS1 antibody (CL488-18703) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CPS1 Polyclonal antibody

CPS1 Polyclonal Antibody for IF/ICC
Cat No. CL488-18703

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

CPS1, CPSase I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CPS1 Ready-To-Use IHC Kit. CPS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18703-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18703 targets CPS1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbamoyl-phosphate synthetase 1, mitochondrial
Synonyms CPS1, CPSase I
Calculated Molecular Weight 165 kDa
Observed Molecular Weight140-165 kDa
GenBank Accession NumberBC140943
Gene Symbol CPS1
Gene ID (NCBI) 1373
RRIDAB_3672699
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31327
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPS1 is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. Expressed primarily in the liver and small intestine, CPS1 can be used as a marker for mitochondria in these tissues. In addition, CPS1 expression appears to be lost in adenocarcinomas of the small intestine. The antibody can recognize two isoforms of this gene around 165 kDa and 116 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CPS1 antibody CL488-18703Download protocol
Standard Protocols
Click here to view our Standard Protocols