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验证数据展示

  • WB analysis using 66039-3-Ig

    WB analysis using 66039-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 66039-3-Ig

    IHC staining of mouse heart using 66039-3-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPT1A antibody included
  • IHC staining of mouse heart using 66039-3-Ig

    IHC staining of mouse heart using 66039-3-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPT1A antibody included
  • IHC staining of rat heart using 66039-3-Ig

    IHC staining of rat heart using 66039-3-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPT1A antibody included
  • IHC staining of rat heart using 66039-3-Ig

    IHC staining of rat heart using 66039-3-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPT1A antibody included
  • WB analysis of mouse liver using 66039-3-Ig

    WB analysis of mouse liver using 66039-3-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66039-3-Ig

    WB analysis using 66039-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66039-3-Ig

    WB analysis using 66039-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 66039-3-Ig

    IHC staining of mouse skeletal muscle using 66039-3-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPT1A antibody included
  • IHC staining of mouse skeletal muscle using 66039-3-Ig

    IHC staining of mouse skeletal muscle using 66039-3-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPT1A antibody included

CPT1A Monoclonal antibody

CPT1A Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66039-3-Ig

产品说明书

CloneNo. 5D2B8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CPT-1A, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform, Carnitine O-palmitoyltransferase 1, liver isoform

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTT cells, HeLa cells, rat liver tissue, mouse liver tissue, LNCaP cells, PANC-1 cells, HaCaT cells
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CPT1A Ready-To-Use IHC Kit. CPT1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66039-3-Ig targets CPT1A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CPT1A fusion protein Ag7745 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 carnitine palmitoyltransferase 1A (liver)
别名 CPT-1A, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform, Carnitine O-palmitoyltransferase 1, liver isoform
计算分子量 88 kDa
观测分子量86 kDa
GenBank蛋白编号BC000185
基因名称 CPT1A
Gene ID (NCBI) 1374
RRIDAB_3670374
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50416
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

实验方案

Product Specific Protocols
WB protocol for CPT1A antibody 66039-3-IgDownload protocol
IHC protocol for CPT1A antibody 66039-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols