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CPT1B-specific Polyclonal antibody
CPT1B-specific Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy CPT1B Ready-To-Use IHC Kit. CPT1B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22170-1-AP targets CPT1B-specific in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish, sheep, ewes |
| Immunogen | CPT1B-specific fusion protein Ag17840 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | carnitine palmitoyltransferase 1B (muscle) |
| Synonyms | CPT1B, CPT1 M, Carnitine palmitoyltransferase I-like protein, Carnitine O-palmitoyltransferase I, muscle isoform, Carnitine O-palmitoyltransferase 1, muscle isoform |
| Calculated Molecular Weight | 567 aa, 64 kDa |
| Observed Molecular Weight | 75-85 kDa |
| GenBank Accession Number | BC131570 |
| Gene Symbol | CPT1B |
| Gene ID (NCBI) | 1375 |
| RRID | AB_2713959 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92523 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CPT1B-specific antibody 22170-1-AP | Download protocol |
| IHC protocol for CPT1B-specific antibody 22170-1-AP | Download protocol |
| IF protocol for CPT1B-specific antibody 22170-1-AP | Download protocol |
| IP protocol for CPT1B-specific antibody 22170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis. | ||
Cell Metab Global Analysis of Plasma Lipids Identifies Liver-Derived Acylcarnitines as a Fuel Source for Brown Fat Thermogenesis.
| ||
Adv Sci (Weinh) m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia | ||
Nat Commun Mechanism of action and therapeutic route for a muscular dystrophy caused by a genetic defect in lipid metabolism. | ||
J Biomed Sci Adipocyte pyroptosis occurs in omental tumor microenvironment and is associated with chemoresistance of ovarian cancer | ||
Biomaterials LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells. |
