验证数据展示
经过测试的应用
Positive IF-P detected in | mouse heart tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-22170 targets CPT1B-specific in IF-P applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF-P Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CPT1B-specific fusion protein Ag17840 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | carnitine palmitoyltransferase 1B (muscle) |
别名 | CPT I, CPT1 M, CPT1B, CPTI M, FLJ55729, FLJ58750, KIAA1670, M CPT1, MCCPT1 |
计算分子量 | 567 aa, 64 kDa |
观测分子量 | 75-85 kDa |
GenBank蛋白编号 | BC131570 |
基因名称 | CPT1B |
Gene ID (NCBI) | 1375 |
RRID | AB_2923748 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q92523 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CPT1B-specific antibody CL488-22170 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |