验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy CPT1B Ready-To-Use IHC Kit. CPT1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22170-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-22170 targets CPT1B-specific in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | CPT1B-specific fusion protein Ag17840 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | carnitine palmitoyltransferase 1B (muscle) |
| Synonyms | CPT I, CPT1 M, CPT1B, CPTI M, FLJ55729, FLJ58750, KIAA1670, M CPT1, MCCPT1 |
| Calculated Molecular Weight | 567 aa, 64 kDa |
| Observed Molecular Weight | 75-85 kDa |
| GenBank Accession Number | BC131570 |
| Gene Symbol | CPT1B |
| Gene ID (NCBI) | 1375 |
| RRID | AB_2923748 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92523 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CPT1B-specific antibody CL488-22170 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |