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  • Featured Product
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CRABP2 Polyclonal antibody

CRABP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10225-1-AP

Print datasheet

Synonyms

CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II

验证数据展示

  • IHC staining of human skin using 10225-1-AP

    IHC staining of human skin using 10225-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 10225-1-AP (CRABP2 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of human skin cancer using 10225-1-AP

    IF Staining of human skin cancer using 10225-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (10225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 10225-1-AP

    IF Staining of MCF-7 using 10225-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CRABP2 antibody (10225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • WB analysis of HeLa using 10225-1-AP

    WB analysis of HeLa using 10225-1-AP

    WB result of CRABP2 antibody (10225-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HeLa cells.

  • IP experiment of HeLa using 10225-1-AP

    IP experiment of HeLa using 10225-1-AP

    IP result of anti-CRABP2 (IP:10225-1-AP, 3ug; Detection:10225-1-AP 1:300) with HeLa cells lysate 4650ug.

  • WB analysis using 10225-1-AP

    WB analysis using 10225-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10225-1-AP (CRABP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 10225-1-AP

    FC experiment of MCF-7 using 10225-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CRABP2 (10225-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HT-29 cells, MCF-7 cells, mouse embryo tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman skin cancer tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10225-1-AP targets CRABP2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CRABP2 fusion protein Ag0309 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cellular retinoic acid binding protein 2
Synonyms CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC001109
Gene Symbol CRABP2
Gene ID (NCBI) 1382
RRIDAB_2085455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29373
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

实验方案

Product Specific Protocols
WB protocol for CRABP2 antibody 10225-1-APDownload protocol
IHC protocol for CRABP2 antibody 10225-1-APDownload protocol
IF protocol for CRABP2 antibody 10225-1-APDownload protocol
IP protocol for CRABP2 antibody 10225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Science

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Authors - David P Doupé
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humanWB

Mol Cell

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humanWB,IF

Neuron

Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles.

Authors - Alana I Mendelsohn
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mouseIF

Nat Commun

The developing mouse coronal suture at single-cell resolution.

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mouseIF

Dev Cell

Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.

Authors - John DeSisto
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humanIF

Stem Cell Reports

BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions.

Authors - Kai Kretzschmar
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