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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CRABP2 Polyclonal antibody

CRABP2 Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10225

Print datasheet

Synonyms

CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II

验证数据展示

  • IF Staining of human skin cancer using CL594-10225

    IF Staining of human skin cancer using CL594-10225

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CoraLite®594-conjugated CRABP2 antibody (CL594-10225) at dilution of 1:50.

  • IF Staining of MCF-7 using CL594-10225

    IF Staining of MCF-7 using CL594-10225

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 CRABP2 antibody (CL594-10225) at dilution of 1:200.

  • FC experiment of MCF-7 using CL594-10225

    FC experiment of MCF-7 using CL594-10225

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CRABP2 (CL594-10225) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF-P detected inhuman skin cancer tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 10225-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10225 targets CRABP2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CRABP2 fusion protein Ag0309 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cellular retinoic acid binding protein 2
Synonyms CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II
Calculated Molecular Weight 16 kDa
GenBank Accession NumberBC001109
Gene Symbol CRABP2
Gene ID (NCBI) 1382
RRIDAB_2883404
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29373
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

实验方案

Product Specific Protocols
IF protocol for CL594 CRABP2 antibody CL594-10225Download protocol
Standard Protocols
Click here to view our Standard Protocols