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验证数据展示

  • IF Staining of MCF-7 using CL488-66468

    IF Staining of MCF-7 using CL488-66468

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 CRABP2 antibody (CL488-66468, Clone: 1A5F3 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of MCF-7 using CL488-66468

    FC experiment of MCF-7 using CL488-66468

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CRABP2 (CL488-66468, Clone:1A5F3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CRABP2 Monoclonal antibody

CRABP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66468

产品说明书

CloneNo. 1A5F3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66468-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66468 targets CRABP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CRABP2 fusion protein Ag0309 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cellular retinoic acid binding protein 2
别名 CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II
计算分子量 16 kDa
观测分子量 14 kDa
GenBank蛋白编号BC001109
基因名称 CRABP2
Gene ID (NCBI) 1382
RRIDAB_3084249
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP29373
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CRABP2 antibody CL488-66468Download protocol
Standard Protocols
Click here to view our Standard Protocols