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CRB2 Polyclonal antibody

CRB2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 28015-1-AP

Print datasheet

Synonyms

CRB2, Crumbs homolog 2, crumbs homolog 2 (Drosophila), Crumbs like protein 2, FLJ16786, FLJ38464

验证数据展示

  • IHC staining of mouse brain using 28015-1-AP

    IHC staining of mouse brain using 28015-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28015-1-AP (CRB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28015-1-AP targets CRB2 in IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen CRB2 fusion protein Ag27826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name crumbs homolog 2 (Drosophila)
Synonyms CRB2, Crumbs homolog 2, crumbs homolog 2 (Drosophila), Crumbs like protein 2, FLJ16786, FLJ38464
Calculated Molecular Weight 134 aa
GenBank Accession NumberNM_173689
Gene Symbol CRB2
Gene ID (NCBI) 286204
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5IJ48
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRB2 (Crumbs homolog-2) has a large extracellular domain with epidermal growth-factor-like and laminin-A globular domains, a single transmembrane domain, and an intracellular C-terminal domain of 37 amino acids (PMID: 31438467). CRB2 is a candidate modifying gene of human CRB1-related retinal dystrophy (PMID: 24565864).

实验方案

Product Specific Protocols
IHC protocol for CRB2 antibody 28015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols