验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Caco-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66336 targets CRBN in IF/ICC applications and shows reactivity with human, mouse, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, pig |
| 免疫原 | CRBN fusion protein Ag17166 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cereblon |
| 别名 | 2A9D11, AD-006, cereblon, Protein cereblon |
| 计算分子量 | 442 aa, 51 kDa |
| 观测分子量 | 51-60 kDa |
| GenBank蛋白编号 | BC017419 |
| 基因名称 | CRBN |
| Gene ID (NCBI) | 51185 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96SW2 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks (PMID: 21232561).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CRBN antibody CL488-66336 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |