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CREB1 Monoclonal antibody
CREB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1E11C1
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | human prostate cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:4000-1:16000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
产品信息
67927-1-Ig targets CREB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse |
Immunogen | CREB1 fusion protein Ag2852 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | cAMP responsive element binding protein 1 |
Synonyms | CREB, CREB 1, CREB1 |
Calculated Molecular Weight | 341 aa, 35 kDa |
Observed Molecular Weight | 43-46 kDa |
GenBank Accession Number | BC010636 |
Gene Symbol | CREB1 |
Gene ID (NCBI) | 1385 |
RRID | AB_2918679 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P16220 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CREB1 antibody 67927-1-Ig | Download protocol |
IHC protocol for CREB1 antibody 67927-1-Ig | Download protocol |
IF protocol for CREB1 antibody 67927-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Free Radic Biol Med Reprimo (RPRM) mediates neuronal ferroptosis via CREB-Nrf2/SCD1 pathways in radiation-induced brain injury | ||
ACS Chem Neurosci Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus | ||
BMC Cancer Identification of TLN1 as a prognostic biomarker to effect cell proliferation and differentiation in acute myeloid leukemia |