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CREB3L1,OASIS Monoclonal antibody

CREB3L1,OASIS Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2B5B4

Cat no : 67617-1-Ig

Print datasheet

Synonyms

CREB3L1, OASIS, Old astrocyte specifically-induced substance, Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1

验证数据展示

  • WB analysis using 67617-1-Ig

    WB analysis using 67617-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of HepG2 using 67617-1-Ig

    FC experiment of HepG2 using 67617-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CREB3L1,OASIS (67617-1-Ig, Clone:2B5B4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 67617-1-Ig

    WB analysis using 67617-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, A375 cells, A549 cells, 4T1 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CREB3L1 Ready-To-Use IHC Kit. CREB3L1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67617-1-Ig targets CREB3L1,OASIS in WB, ELISA, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA, FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen CREB3L1,OASIS fusion protein Ag28042 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cAMP responsive element binding protein 3-like 1
Synonyms CREB3L1, OASIS, Old astrocyte specifically-induced substance, Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC015781
Gene Symbol CREB3L1/OASIS
Gene ID (NCBI) 90993
RRIDAB_2882820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96BA8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

实验方案

Product Specific Protocols
WB protocol for CREB3L1,OASIS antibody 67617-1-IgDownload protocol
FC protocol for CREB3L1,OASIS antibody 67617-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols