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CREB3L1,OASIS Monoclonal antibody, PBS Only (Capture)

CREB3L1,OASIS Monoclonal Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B5B4

Cat no : 67617-1-PBS

Print datasheet

Synonyms

CREB3L1, OASIS, Old astrocyte specifically-induced substance, Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1

验证数据展示

  • WB analysis using 67617-1-Ig (same clone as 67617-1-PBS)

    WB analysis using 67617-1-Ig (same clone as 67617-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67617-1-Ig (same clone as 67617-1-PBS)

    FC experiment of HepG2 using 67617-1-Ig (same clone as 67617-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CREB3L1,OASIS (67617-1-Ig, Clone:2B5B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

  • WB analysis using 67617-1-Ig (same clone as 67617-1-PBS)

    WB analysis using 67617-1-Ig (same clone as 67617-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50829-2

    Cytometric bead array standard curve of MP50829-2

    Cytometric bead array standard curve of MP50829-2, CREB3L1,OASIS Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67617-1-PBS. Detection antibody: 67617-4-PBS. Standard:Ag28042. Range: 1.563-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CREB3L1 Ready-To-Use IHC Kit. CREB3L1 primary antibody included.

产品信息

67617-1-PBS targets CREB3L1,OASIS as part of a matched antibody pair:

MP50829-2: 67617-1-PBS capture and 67617-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CREB3L1,OASIS fusion protein Ag28042 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cAMP responsive element binding protein 3-like 1
Synonyms CREB3L1, OASIS, Old astrocyte specifically-induced substance, Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC015781
Gene Symbol CREB3L1/OASIS
Gene ID (NCBI) 90993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96BA8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)