验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
22277-1-AP targets CREBBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CREB binding protein |
Synonyms | RSTS, KAT3A, CBP/p300, CBP |
Calculated Molecular Weight | 265 kDa |
Observed Molecular Weight | 290 kDa |
GenBank Accession Number | NM_004380 |
Gene Symbol | CREBBP |
Gene ID (NCBI) | 1387 |
RRID | AB_3085692 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q92793 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The acetyltransferase CREB-binding protein (CREBBP; also known as CBP or KAT2A) and E1A-binding protein P300 (EP300; also known as P300 or KAT2B) are two closely related regulators. Both are widely expressed within and outside the hematopoietic system and serve as tumor activators or suppressors depending on the situation. CREBBP/EP300 regulate various key physiological functions, including cell apoptosis, proliferation and differentiation, DNA repair, and somatic cell reprogramming.
实验方案
Product Specific Protocols | |
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WB protocol for CREBBP antibody 22277-1-AP | Download protocol |
IHC protocol for CREBBP antibody 22277-1-AP | Download protocol |
IF protocol for CREBBP antibody 22277-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Biosci (Landmark Ed) Exploration of the Pharmacological Mechanism of Vitexicarpin against Triple-Negative Breast Cancer in Network Pharmacology | ||
J Virol Zinc-finger CCHC-type containing protein 8 promotes RNA virus replication by suppressing the type-I interferon responses |