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CREBBP Polyclonal antibody

CREBBP Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22277-1-AP

Print datasheet

Synonyms

CBP, CREB binding protein, CREBBP, KAT3A, RSTS

验证数据展示

  • WB analysis using 22277-1-AP

    WB analysis using 22277-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22277-1-AP (CREBBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 22277-1-AP

    IHC staining of human prostate cancer using 22277-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22277-1-AP (CREBBP antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CREBBP antibody included
  • IHC staining of human prostate cancer using 22277-1-AP

    IHC staining of human prostate cancer using 22277-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22277-1-AP (CREBBP antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CREBBP antibody included
  • IF Staining of HeLa using 22277-1-AP

    IF Staining of HeLa using 22277-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREBBP antibody (22277-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CREBBP Ready-To-Use IHC Kit. CREBBP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22277-1-AP targets CREBBP in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CREB binding protein
Synonyms CBP, CREB binding protein, CREBBP, KAT3A, RSTS
Calculated Molecular Weight 265 kDa
Observed Molecular Weight290 kDa
GenBank Accession NumberNM_004380
Gene Symbol CREBBP
Gene ID (NCBI) 1387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92793
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The acetyltransferase CREB-binding protein (CREBBP; also known as CBP or KAT2A) and E1A-binding protein P300 (EP300; also known as P300 or KAT2B) are two closely related regulators. Both are widely expressed within and outside the hematopoietic system and serve as tumor activators or suppressors depending on the situation. CREBBP/EP300 regulate various key physiological functions, including cell apoptosis, proliferation and differentiation, DNA repair, and somatic cell reprogramming.

实验方案

Product Specific Protocols
WB protocol for CREBBP antibody 22277-1-APDownload protocol
IHC protocol for CREBBP antibody 22277-1-APDownload protocol
IF protocol for CREBBP antibody 22277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols