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CoraLite® Plus 488-conjugated CRM1 Monoclonal antibody

CRM1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D3B6

Cat no : CL488-66763

Print datasheet

Synonyms

XPO1, Exportin-1, Exportin 1, Exp1, 2D3B6

验证数据展示

  • WB analysis using CL488-66763

    WB analysis using CL488-66763

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66763 (CRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy CRM1 Ready-To-Use IHC Kit. CRM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66763-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66763 targets CRM1 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat
Immunogen CRM1 fusion protein Ag27337 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name exportin 1 (CRM1 homolog, yeast)
Synonyms XPO1, Exportin-1, Exportin 1, Exp1, 2D3B6
Calculated Molecular Weight 1071 aa, 123 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC032847
Gene Symbol CRM1
Gene ID (NCBI) 7514
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14980
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 CRM1 antibody CL488-66763Download protocol
Standard Protocols
Click here to view our Standard Protocols