• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using CL488-66763

    WB analysis using CL488-66763

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66763 (CRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated CRM1 Monoclonal antibody

CRM1 Monoclonal Antibody for WB
Cat No. CL488-66763

产品说明书

CloneNo. 2D3B6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB

别名

XPO1, Exportin-1, Exportin 1, Exp1, 2D3B6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy CRM1 Ready-To-Use IHC Kit. CRM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66763-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66763 targets CRM1 in WB applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat
免疫原 CRM1 fusion protein Ag27337 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 exportin 1 (CRM1 homolog, yeast)
别名 XPO1, Exportin-1, Exportin 1, Exp1, 2D3B6
计算分子量 1071 aa, 123 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC032847
基因名称 CRM1
Gene ID (NCBI) 7514
RRIDAB_3084271
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO14980
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 CRM1 antibody CL488-66763Download protocol
Standard Protocols
Click here to view our Standard Protocols