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CoraLite® Plus 488-conjugated CRMP5 Monoclonal antibody

CRMP5 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F2D8

Cat no : CL488-68272

Print datasheet

Synonyms

验证数据展示

  • IF Staining of SH-SY5Y using CL488-68272

    IF Staining of SH-SY5Y using CL488-68272

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 CRMP5 antibody (CL488-68272, Clone: 3F2D8 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 68272-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68272 targets CRMP5 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen CRMP5 fusion protein Ag0783 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name dihydropyrimidinase-like 5
Synonyms
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC002874
Gene Symbol CRMP5
Gene ID (NCBI) 56896
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BPU6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collapsin response mediator proteins (CRMPs) are a family consisting of five homologous cytosolic phosphoproteins (CRMP1-5). All five CRMPs are abundantly expressed in the developing brain and the adult nervous system. CRMP5 is phylogenetically divergent from the other four CRMPs, sharing only 50% homology; this protein plays an important role in modulating filopodial dynamics and, in turn, restricting neuronal growth cone development.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CRMP5 antibody CL488-68272Download protocol
Standard Protocols
Click here to view our Standard Protocols