验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83914-1-RR targets CRNKL1 in IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | crooked neck pre-mRNA splicing factor-like 1 (Drosophila) |
| 别名 | HCRN, Crooked neck like protein 1, Crooked neck homolog, CRN, CLF |
| 计算分子量 | 100 kDa |
| GenBank蛋白编号 | NM_016652 |
| 基因名称 | CRNKL1 |
| Gene ID (NCBI) | 51340 |
| RRID | AB_3671494 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9BZJ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRNKL1 (Crooked Neck Pre-MRNA Splicing Factor 1) is involved in pre-mRNA splicing process. As a component of the minor spliceosome, it's involved in the splicing of U12-type introns in pre-mRNAs. It's reported that CRNKL1 is a nuclear retention factor of the HIV-1 unspliced RNA and a selective regulator of cytoplasmic levels of a subset of intron-retaining cellular mRNAs.(PMID: 33468685)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CRNKL1 antibody 83914-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |