验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 83914-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83914 targets CRNKL1 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | crooked neck pre-mRNA splicing factor-like 1 (Drosophila) |
| 别名 | |
| 计算分子量 | 100 kDa |
| GenBank蛋白编号 | NM_016652 |
| 基因名称 | CRNKL1 |
| Gene ID (NCBI) | 51340 |
| RRID | AB_3673325 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9BZJ0 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRNKL1 (Crooked Neck Pre-MRNA Splicing Factor 1) is involved in pre-mRNA splicing process. As a component of the minor spliceosome, it's involved in the splicing of U12-type introns in pre-mRNAs. It's reported that CRNKL1 is a nuclear retention factor of the HIV-1 unspliced RNA and a selective regulator of cytoplasmic levels of a subset of intron-retaining cellular mRNAs.(PMID: 33468685)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CRNKL1 antibody CL488-83914 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |