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验证数据展示

  • WB analysis using 12497-1-AP (same clone as 12497-1-PBS)

    WB analysis using 12497-1-AP (same clone as 12497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 12497-1-AP (same clone as 12497-1-PBS)

    IHC staining of human skin cancer using 12497-1-AP (same clone as 12497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CRTC2 antibody included
  • IF Staining of HepG2 using 12497-1-AP (same clone as 12497-1-PBS)

    IF Staining of HepG2 using 12497-1-AP (same clone as 12497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CRTC2,TORC2 antibody (12497-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TORC2 Polyclonal antibody, PBS Only

TORC2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12497-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CRTC2,TORC2, CRTC2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2, TORC 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CRTC2 Ready-To-Use IHC Kit. CRTC2 primary antibody included.

产品信息

12497-1-PBS targets TORC2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TORC2 fusion protein Ag3167 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CREB regulated transcription coactivator 2
别名 CRTC2,TORC2, CRTC2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2, TORC 2
计算分子量 693 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC053562
基因名称 TORC2
Gene ID (NCBI) 200186
RRIDAB_2260910
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53ET0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .