• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CRTC2,TORC2 Polyclonal antibody, PBS Only

CRTC2,TORC2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12497-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 12497-1-AP (same clone as 12497-1-PBS)

    WB analysis using 12497-1-AP (same clone as 12497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12497-1-AP (same clone as 12497-1-PBS)

    IF Staining of HeLa using 12497-1-AP (same clone as 12497-1-PBS)

    Immunofluorescent analysis of Hela cells, using CRTC2 antibody 12497-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 12497-1-AP (same clone as 12497-1-PBS)

    IHC staining of human skin cancer using 12497-1-AP (same clone as 12497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CRTC2 antibody included
Planning an IHC experiment? We recommend our IHCeasy CRTC2 Ready-To-Use IHC Kit. CRTC2 primary antibody included.

产品信息

12497-1-PBS targets CRTC2,TORC2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CRTC2,TORC2 fusion protein Ag3167 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CREB regulated transcription coactivator 2
Synonyms
Calculated Molecular Weight 693 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC053562
Gene Symbol TORC2
Gene ID (NCBI) 200186
RRIDAB_2260910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53ET0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .