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CRX Monoclonal antibody

CRX Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C3C11

Cat no : 67555-1-Ig

Print datasheet

Synonyms

cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, LCA7, OTX3

验证数据展示

  • WB analysis using 67555-1-Ig

    WB analysis using 67555-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67555-1-Ig (CRX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 67555-1-Ig

    IHC staining of mouse eye using 67555-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67555-1-Ig (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CRX antibody included

经过测试的应用

Positive WB detected inrat retina tissue, Y79 cells, mouse retina tissue, pig retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CRX Ready-To-Use IHC Kit. CRX primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67555-1-Ig targets CRX in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen CRX fusion protein Ag30086 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cone-rod homeobox
Synonyms cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, LCA7, OTX3
Calculated Molecular Weight 299 aa, 32 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC016664
Gene Symbol CRX
Gene ID (NCBI) 1406
RRIDAB_2882769
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43186
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cone-rod homeobox (Crx) is a homeodomain transcription factor and member of the Otx family, which has been thought to play a critical role in determining and maintaining the phenotype of both pinealocytes and retinal photoreceptors [PMID:17467693]. In the mammalian retina, Crx plays an essential role in the normal development and maintenance of cones and rods and regulates expression of the network of genes that characterize the retina [PMID:17653270]. Elimination of Crx by disruption of the homeobox domain results in loss of the image-forming visual system but not the non-image-forming visual system controlling circadian rhythms [PMID:20438719].

实验方案

Product Specific Protocols
WB protocol for CRX antibody 67555-1-IgDownload protocol
IHC protocol for CRX antibody 67555-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols