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验证数据展示

  • WB analysis using 67555-1-Ig

    WB analysis using 67555-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67555-1-Ig (CRX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 67555-1-Ig

    IHC staining of mouse eye using 67555-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67555-1-Ig (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CRX antibody included

CRX Monoclonal antibody

CRX Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67555-1-Ig

产品说明书

CloneNo. 1C3C11

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, LCA7, OTX3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat retina tissue, Y79 cells, mouse retina tissue, pig retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CRX Ready-To-Use IHC Kit. CRX primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67555-1-Ig targets CRX in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 CRX fusion protein Ag30086 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cone-rod homeobox
别名 cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, LCA7, OTX3
计算分子量 299 aa, 32 kDa
观测分子量 37 kDa
GenBank蛋白编号BC016664
基因名称 CRX
Gene ID (NCBI) 1406
RRIDAB_2882769
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43186
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cone-rod homeobox (Crx) is a homeodomain transcription factor and member of the Otx family, which has been thought to play a critical role in determining and maintaining the phenotype of both pinealocytes and retinal photoreceptors [PMID:17467693]. In the mammalian retina, Crx plays an essential role in the normal development and maintenance of cones and rods and regulates expression of the network of genes that characterize the retina [PMID:17653270]. Elimination of Crx by disruption of the homeobox domain results in loss of the image-forming visual system but not the non-image-forming visual system controlling circadian rhythms [PMID:20438719].

实验方案

Product Specific Protocols
WB protocol for CRX antibody 67555-1-IgDownload protocol
IHC protocol for CRX antibody 67555-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols