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Placental lactogen Recombinant antibody

Placental lactogen Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241625E1

Cat no : 84361-2-RR

Print datasheet

Synonyms

CSH1, PL, CSH2, Chorionic somatomammotropin hormone 2, Choriomammotropin Alternative names Lactogen

验证数据展示

  • WB analysis of human placenta using 84361-2-RR

    WB analysis of human placenta using 84361-2-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84361-2-RR (CSH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 84361-2-RR

    IHC staining of human placenta using 84361-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84361-2-RR (Placental lactogen antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 84361-2-RR

    IHC staining of human placenta using 84361-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84361-2-RR (Placental lactogen antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84361-2-RR

    Affinity and Kinetic Characterization of 84361-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84361-2-RR against Human Placental lactogen were performed. The affinity constant is 2.58 nM.

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84361-2-RR targets Placental lactogen in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Placental lactogen fusion protein Ag0201 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chorionic somatomammotropin hormone 1 (placental lactogen)
Synonyms CSH1, PL, CSH2, Chorionic somatomammotropin hormone 2, Choriomammotropin Alternative names Lactogen
Calculated Molecular Weight 25 kDa
Observed Molecular Weight20-25 kDa
GenBank Accession NumberBC002717
Gene Symbol CSH1
Gene ID (NCBI) 1442
RRIDAB_3671899
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP0DML2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chorionic somatomammotropin hormone 1 (placental lactogen, CSH1), is a hormone with the molecular weight of 25 kDa. The data suggest that this protein may link with the Silver-Russell syndrome (SRS), a disease of pre- and postnatal growth restriction and a characteristic small, triangular face. SRS is also accompanied by other dysmorphic features including fifth finger clinodactyly and skeletal asymmetry.

实验方案

Product Specific Protocols
WB protocol for Placental lactogen antibody 84361-2-RRDownload protocol
IHC protocol for Placental lactogen antibody 84361-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols