验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, rabbit heart tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells, HL-60 cells |
| Positive IF/ICC detected in | A431 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68200-1-Ig targets CSNK2A1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, rabbit |
| 文献引用反应性 | human |
| 免疫原 | CSNK2A1 fusion protein Ag29062 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | casein kinase 2, alpha 1 polypeptide |
| 别名 | CKII, CK2α, CK2A1, CK2a, CK II alpha |
| 计算分子量 | 45 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC011668 |
| 基因名称 | CSNK2A1 |
| Gene ID (NCBI) | 1457 |
| RRID | AB_2935289 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P68400 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSNK2A1 antibody 68200-1-Ig | Download protocol |
| IF protocol for CSNK2A1 antibody 68200-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
