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CSNK2A1 Monoclonal antibody

CSNK2A1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D5E8

Cat no : 68200-1-Ig

Print datasheet

Synonyms

CKII, CK2α, CK2A1, CK2a, CK II alpha

验证数据展示

  • WB analysis using 68200-1-Ig

    WB analysis using 68200-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of A431 using 68200-1-Ig

    IF Staining of A431 using 68200-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inLNCaP cells, rabbit heart tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells, HL-60 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CSNK2A1 Ready-To-Use IHC Kit. CSNK2A1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68200-1-Ig targets CSNK2A1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rabbit samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, rabbit
Immunogen CSNK2A1 fusion protein Ag29062 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name casein kinase 2, alpha 1 polypeptide
Synonyms CKII, CK2α, CK2A1, CK2a, CK II alpha
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC011668
Gene Symbol CSNK2A1
Gene ID (NCBI) 1457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP68400
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

实验方案

Product Specific Protocols
WB protocol for CSNK2A1 antibody 68200-1-IgDownload protocol
IF protocol for CSNK2A1 antibody 68200-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols