验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CSPP1 Ready-To-Use IHC Kit. CSPP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11931-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-11931 targets CSPP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | CSPP1 fusion protein Ag2521 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrosome and spindle pole associated protein 1 |
| 别名 | CSPP, CSPP1, FLJ22490, FLJ38886 |
| 计算分子量 | 141 kDa |
| 观测分子量 | 141 kDa |
| GenBank蛋白编号 | BC029445 |
| 基因名称 | CSPP1 |
| Gene ID (NCBI) | 79848 |
| RRID | AB_2919043 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q1MSJ5 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CSPP1 antibody CL488-11931 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |