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CSRP1 Polyclonal antibody

CSRP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13432-1-AP

Print datasheet

Synonyms

Cysteine rich protein 1, Cysteine and glycine-rich protein 1, CYRP, CSRP, CRP1

验证数据展示

  • WB analysis of human stomach using 13432-1-AP

    WB analysis of human stomach using 13432-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 13432-1-AP (CSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse stomach using 13432-1-AP

    IP experiment of mouse stomach using 13432-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13432-1-AP (CSRP1 antibody) at dilution of 1:300.

  • IHC staining of human normal colon using 13432-1-AP

    IHC staining of human normal colon using 13432-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13432-1-AP (CSRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human normal colon using 13432-1-AP

    IHC staining of human normal colon using 13432-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13432-1-AP (CSRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman stomach tissue
Positive IP detected inmouse stomach tissue
Positive IHC detected inhuman normal colon, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13432-1-AP targets CSRP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen CSRP1 fusion protein Ag4236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cysteine and glycine-rich protein 1
Synonyms Cysteine rich protein 1, Cysteine and glycine-rich protein 1, CYRP, CSRP, CRP1
Calculated Molecular Weight 193 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC032493
Gene Symbol CSRP1
Gene ID (NCBI) 1465
RRIDAB_2230180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21291
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CSRP1 antibody 13432-1-APDownload protocol
IHC protocol for CSRP1 antibody 13432-1-APDownload protocol
IP protocol for CSRP1 antibody 13432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Mol Med Rep

Inflammatory and oxidative stress‑associated factors in chronic intermittent hypoxia in Chinese patients, rats, lymphocytes and endotheliocytes.

Authors - Hui Ren
View Article