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CTBP1 Monoclonal antibody, PBS Only

CTBP1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

1C4B2

Cat No : 66995-1-PBS

Print datasheet

Synonyms

BARS, C terminal binding protein 1, CTBP, CTBP1

验证数据展示

  • IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)

    IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTBP1 antibody included
  • WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)

    WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    IF Staining of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    FC experiment of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CTBP1 Ready-To-Use IHC Kit. CTBP1 primary antibody included.

产品信息

66995-1-PBS targets CTBP1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity Human, rat, mouse
Immunogen CTBP1 fusion protein Ag28567 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name C-terminal binding protein 1
Synonyms BARS, C terminal binding protein 1, CTBP, CTBP1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC011655
Gene Symbol CTBP1
Gene ID (NCBI) 1487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13363
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.