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验证数据展示

  • IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)

    IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTBP1 antibody included
  • WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)

    WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    IF Staining of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    FC experiment of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

CTBP1 Monoclonal antibody, PBS Only

CTBP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66995-1-PBS

产品说明书

CloneNo. 1C4B2

宿主/亚型

Mouse / IgG1

种属反应性

rat, mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS, 1C4B2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CTBP1 Ready-To-Use IHC Kit. CTBP1 primary antibody included.

产品信息

66995-1-PBS targets CTBP1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 rat, mouse, human
免疫原 CTBP1 fusion protein Ag28567 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 C-terminal binding protein 1
别名 EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS, 1C4B2
计算分子量 48 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC011655
基因名称 CTBP1
Gene ID (NCBI) 1487
RRIDAB_2882312
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13363
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.