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验证数据展示

  • IF Staining of A431 using CL488-66995

    IF Staining of A431 using CL488-66995

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 CTBP1 antibody (CL488-66995, Clone: 1C4B2 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66995

    FC experiment of HepG2 using CL488-66995

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CTBP1 (CL488-66995, Clone:1C4B2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated CTBP1 Monoclonal antibody

CTBP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66995

产品说明书

CloneNo. 1C4B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS, 1C4B2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells, HeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CTBP1 Ready-To-Use IHC Kit. CTBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66995-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66995 targets CTBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CTBP1 fusion protein Ag28567 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 C-terminal binding protein 1
别名 EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS, 1C4B2
计算分子量 48 kDa
GenBank蛋白编号BC011655
基因名称 CTBP1
Gene ID (NCBI) 1487
RRIDAB_2883394
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13363
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CTBP1 antibody CL488-66995Download protocol
Standard Protocols
Click here to view our Standard Protocols