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CTP synthase Polyclonal antibody

CTP synthase Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 15914-1-AP

Print datasheet

Synonyms

CTPS, UTP--ammonia ligase 1, EC:6.3.4.2, CTPS1, CTPS 1



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15914-1-AP targets CTP synthase in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse, zebrafish, mosquito
Immunogen CTP synthase fusion protein Ag8707 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CTP synthase
Synonyms CTPS, UTP--ammonia ligase 1, EC:6.3.4.2, CTPS1, CTPS 1
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC009408
Gene Symbol CTPS
Gene ID (NCBI) 1503
RRIDAB_2086513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17812
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTP synthase(CTPS) is also named as CTPS1 and belongs to the CTP synthase family. It catalyses the ATP-dependent formation of CTP from UTP using either L-glutamine or NH3 as the nitrogen source(PMID:12752439). It is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis (PMID:8813694). CTP synthetase exists as a dimer in the absence of its substrates ATP and UTP, but in the presence of saturating concentrations of these substrates the enzyme exists as a tetramer.(PMID:18439916)

实验方案

Product Specific Protocols
WB protocol for CTP synthase antibody 15914-1-APDownload protocol
IHC protocol for CTP synthase antibody 15914-1-APDownload protocol
IF protocol for CTP synthase antibody 15914-1-APDownload protocol
IP protocol for CTP synthase antibody 15914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Res

Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.

Authors - Zhe Sun
humanIF

FEBS J

Inter-filament interaction between IMPDH and CTPS cytoophidia.

Authors - Chia-Chun Chang
humanWB,CoIP

J Cell Physiol

Energy deficiency caused by CTPS downregulation in decidua may contribute to pre-eclampsia by impairing decidualization.

Authors - Ke Ma
human

Front Immunol

Flow Cytometry Contributions for the Diagnosis and Immunopathological Characterization of Primary Immunodeficiency Diseases With Immune Dysregulation.

Authors - Otavio Cabral-Marques
humanWB

Oncotarget

Identification and validation of differentially expressed proteins in epithelial ovarian cancers using quantitative proteomics.

Authors - Hong Qu
humanIF

J Cell Sci

Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.

Authors - S John Calise