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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CTP synthase Polyclonal antibody

CTP synthase Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15914

Print datasheet

Synonyms

CTPS, UTP--ammonia ligase 1, EC:6.3.4.2, CTPS1, CTPS 1

验证数据展示

  • IF Staining of HepG2 using CL488-15914

    IF Staining of HepG2 using CL488-15914

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 CTP synthase antibody (CL488-15914) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-15914

    FC experiment of HepG2 using CL488-15914

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CTP synthase (CL488-15914) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 15914-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15914 targets CTP synthase in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen CTP synthase fusion protein Ag8707 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CTP synthase
Synonyms CTPS, UTP--ammonia ligase 1, EC:6.3.4.2, CTPS1, CTPS 1
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC009408
Gene Symbol CTPS
Gene ID (NCBI) 1503
RRIDAB_2919125
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17812
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTP synthase(CTPS) is also named as CTPS1 and belongs to the CTP synthase family. It catalyses the ATP-dependent formation of CTP from UTP using either L-glutamine or NH3 as the nitrogen source(PMID:12752439). It is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis (PMID:8813694). CTP synthetase exists as a dimer in the absence of its substrates ATP and UTP, but in the presence of saturating concentrations of these substrates the enzyme exists as a tetramer.(PMID:18439916)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CTP synthase antibody CL488-15914Download protocol
FC protocol for CL Plus 488 CTP synthase antibody CL488-15914Download protocol
Standard Protocols
Click here to view our Standard Protocols