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CTPS2 Monoclonal antibody

CTPS2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A3B8

Cat no : 67734-1-Ig

Print datasheet

Synonyms

UTP ammonia ligase 2, EC:6.3.4.2, CTP synthetase 2, CTP synthase II, CTP synthase 2

验证数据展示

  • WB analysis using 67734-1-Ig

    WB analysis using 67734-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human prostate cancer using 67734-1-Ig

    IHC staining of human prostate cancer using 67734-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human prostate cancer using 67734-1-Ig

    IF Staining of human prostate cancer using 67734-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of PC-12 using 67734-1-Ig

    IF Staining of PC-12 using 67734-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, U2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Positive IF/ICC detected inPC-12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67734-1-Ig targets CTPS2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CTPS2 fusion protein Ag30258 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CTP synthase II
Synonyms UTP ammonia ligase 2, EC:6.3.4.2, CTP synthetase 2, CTP synthase II, CTP synthase 2
Calculated Molecular Weight 586 aa, 66 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberBC034986
Gene Symbol CTPS2
Gene ID (NCBI) 56474
RRIDAB_2882918
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRF8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.

实验方案

Product Specific Protocols
WB protocol for CTPS2 antibody 67734-1-IgDownload protocol
IHC protocol for CTPS2 antibody 67734-1-IgDownload protocol
IF protocol for CTPS2 antibody 67734-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols