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Cortactin Polyclonal antibody

Cortactin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11381-1-AP

Print datasheet

Synonyms

CTTN, SRC8, EMS1

验证数据展示

  • IHC staining of human breast cancer using 11381-1-AP

    IHC staining of human breast cancer using 11381-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cortactin antibody included
  • IHC staining of human breast cancer using 11381-1-AP

    IHC staining of human breast cancer using 11381-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cortactin antibody included
  • IP experiment of MCF-7 using 11381-1-AP

    IP experiment of MCF-7 using 11381-1-AP

    IP result of anti-Cortactin (IP:11381-1-AP, 4ug; Detection:11381-1-AP 1:1000) with MCF-7 cells lysate 2400 ug.

  • WB analysis using 11381-1-AP

    WB analysis using 11381-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11381-1-AP

    WB analysis using 11381-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11381-1-AP

    WB analysis using 11381-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 11381-1-AP

    IHC staining of mouse colon using 11381-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cortactin antibody included
  • IHC staining of mouse colon using 11381-1-AP

    IHC staining of mouse colon using 11381-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cortactin antibody included
  • IF Staining of HeLa using 11381-1-AP

    IF Staining of HeLa using 11381-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cortactin antibody (11381-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 11381-1-AP

    FC experiment of HeLa using 11381-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cortactin Polyclonal antibody (11381-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, HUVEC cells, HT-1080 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cortactin Ready-To-Use IHC Kit. Cortactin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11381-1-AP targets Cortactin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Cortactin fusion protein Ag1940 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cortactin
Synonyms CTTN, SRC8, EMS1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight70-85 kDa
GenBank Accession NumberBC008799
Gene Symbol Cortactin
Gene ID (NCBI) 2017
RRIDAB_2088125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14247
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

实验方案

Product Specific Protocols
WB protocol for Cortactin antibody 11381-1-APDownload protocol
IHC protocol for Cortactin antibody 11381-1-APDownload protocol
IF protocol for Cortactin antibody 11381-1-APDownload protocol
IP protocol for Cortactin antibody 11381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

J Autoimmun

Alternative splicing and intron retention: Their profiles and roles in cutaneous fibrosis of systemic sclerosis

Authors - Shasha Xie
View Article
mouseIF

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma
View Article
WB

Genes Dev

Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein

Authors - Megan L Norris
View Article
humanIF

Adv Healthc Mater

Fiber Microarchitecture in Interpenetrating Collagen-Alginate Hydrogel with Tunable Mechanical Plasticity Regulates Tumor Cell Migration

Authors - Zhao Wei
View Article
humanWB

EMBO J

TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.

Authors - Qichao Huang
View Article
humanIHC

Front Oncol

A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma

Authors - Hao Wang
View Article