验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells, HUVEC cells, HT-1080 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11381-1-AP targets Cortactin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Cortactin fusion protein Ag1940 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cortactin |
| 别名 | CTTN, SRC8, EMS1 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 70-85 kDa |
| GenBank蛋白编号 | BC008799 |
| 基因名称 | Cortactin |
| Gene ID (NCBI) | 2017 |
| RRID | AB_2088125 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14247 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cortactin antibody 11381-1-AP | Download protocol |
| IHC protocol for Cortactin antibody 11381-1-AP | Download protocol |
| IF protocol for Cortactin antibody 11381-1-AP | Download protocol |
| IP protocol for Cortactin antibody 11381-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Autoimmun Alternative splicing and intron retention: Their profiles and roles in cutaneous fibrosis of systemic sclerosis | ||
Circ. Res. Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm. | ||
Genes Dev Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein | ||
Adv Healthc Mater Fiber Microarchitecture in Interpenetrating Collagen-Alginate Hydrogel with Tunable Mechanical Plasticity Regulates Tumor Cell Migration | ||
EMBO J TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis. | ||
Front Oncol A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma |