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CoraLite® Plus 647-conjugated Cortactin Polyclonal antibody

Cortactin Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-11381

Print datasheet

Synonyms

Amplaxin, cortactin, CTTN, EMS1, FLJ34459, Oncogene EMS1, Src substrate cortactin

验证数据展示

  • FC experiment of HeLa using CL647-11381

    FC experiment of HeLa using CL647-11381

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cortactin (CL647-11381) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cortactin Ready-To-Use IHC Kit. Cortactin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11381-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-11381 targets Cortactin in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Cortactin fusion protein Ag1940 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cortactin
Synonyms Amplaxin, cortactin, CTTN, EMS1, FLJ34459, Oncogene EMS1, Src substrate cortactin
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 80 kDa, 85 kDa
GenBank Accession NumberBC008799
Gene Symbol Cortactin
Gene ID (NCBI) 2017
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14247
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Cortactin antibody CL647-11381Download protocol
Standard Protocols
Click here to view our Standard Protocols