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验证数据展示

  • FC experiment of HeLa using CL647-11381

    FC experiment of HeLa using CL647-11381

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cortactin (CL647-11381) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated Cortactin Polyclonal antibody

Cortactin Polyclonal Antibody for FC (Intra)
Cat No. CL647-11381

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

Amplaxin, cortactin, CTTN, EMS1, FLJ34459, Oncogene EMS1, Src substrate cortactin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cortactin Ready-To-Use IHC Kit. Cortactin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11381-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-11381 targets Cortactin in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Cortactin fusion protein Ag1940 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cortactin
别名 Amplaxin, cortactin, CTTN, EMS1, FLJ34459, Oncogene EMS1, Src substrate cortactin
计算分子量 57 kDa
观测分子量 80 kDa, 85 kDa
GenBank蛋白编号BC008799
基因名称 Cortactin
Gene ID (NCBI) 2017
RRIDAB_2934856
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14247
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Cortactin antibody CL647-11381Download protocol
Standard Protocols
Click here to view our Standard Protocols