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CUL1 Polyclonal antibody

CUL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28404-1-AP

Print datasheet

Synonyms

CUL 1, CUL1, cullin 1

验证数据展示

  • WB analysis using 28404-1-AP

    WB analysis using 28404-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28404-1-AP (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28404-1-AP

    IHC staining of human breast cancer using 28404-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28404-1-AP (CUL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CUL1 antibody included
  • IHC staining of human breast cancer using 28404-1-AP

    IHC staining of human breast cancer using 28404-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28404-1-AP (CUL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CUL1 antibody included

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CUL1 Ready-To-Use IHC Kit. CUL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28404-1-AP targets CUL1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen CUL1 fusion protein Ag29145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cullin 1
Synonyms CUL 1, CUL1, cullin 1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC034318
Gene Symbol CUL1
Gene ID (NCBI) 8454
RRIDAB_2881135
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13616
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

实验方案

Product Specific Protocols
WB protocol for CUL1 antibody 28404-1-APDownload protocol
IHC protocol for CUL1 antibody 28404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols