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  • Featured Product
  • KD/KO Validated

CUL2 Monoclonal antibody, PBS Only

CUL2 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A6G9

Cat No : 67175-1-PBS

Print datasheet

Synonyms

CUL 2, CUL2, cullin 2

验证数据展示

  • WB analysis of MCF-7 using 67175-1-Ig (same clone as 67175-1-PBS)

    WB analysis of MCF-7 using 67175-1-Ig (same clone as 67175-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • WB analysis of Raji using 67175-1-Ig (same clone as 67175-1-PBS)

    WB analysis of Raji using 67175-1-Ig (same clone as 67175-1-PBS)

    WB result of CUL2 antibody (67175-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected Raji cells. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

    IF Staining of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUL2 antibody (67175-1-Ig, Clone: 1A6G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

产品信息

67175-1-PBS targets CUL2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human, Rat
Immunogen CUL2 fusion protein Ag28654 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cullin 2
Synonyms CUL 2, CUL2, cullin 2
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberBC009591
Gene Symbol CUL2
Gene ID (NCBI) 8453
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13617
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.