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  • Featured Product
  • KD/KO Validated

CUL2 Monoclonal antibody, PBS Only

CUL2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A6G9

Cat no : 67175-1-PBS

Print datasheet

Synonyms

CUL 2, CUL2, cullin 2

验证数据展示

  • WB analysis of MCF-7 using 67175-1-Ig (same clone as 67175-1-PBS)

    WB analysis of MCF-7 using 67175-1-Ig (same clone as 67175-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • WB analysis of Raji using 67175-1-Ig (same clone as 67175-1-PBS)

    WB analysis of Raji using 67175-1-Ig (same clone as 67175-1-PBS)

    WB result of CUL2 antibody (67175-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected Raji cells. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

    IF Staining of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUL2 antibody (67175-1-Ig, Clone: 1A6G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

产品信息

67175-1-PBS targets CUL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Rat
Immunogen CUL2 fusion protein Ag28654 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cullin 2
Synonyms CUL 2, CUL2, cullin 2
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberBC009591
Gene Symbol CUL2
Gene ID (NCBI) 8453
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13617
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.