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验证数据展示

  • WB analysis of MCF-7 using 67175-1-Ig (same clone as 67175-1-PBS)

    WB analysis of MCF-7 using 67175-1-Ig (same clone as 67175-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • WB analysis of Raji using 67175-1-Ig (same clone as 67175-1-PBS)

    WB analysis of Raji using 67175-1-Ig (same clone as 67175-1-PBS)

    WB result of CUL2 antibody (67175-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected Raji cells. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • WB analysis using 67175-1-Ig (same clone as 67175-1-PBS)

    WB analysis using 67175-1-Ig (same clone as 67175-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL2 antibody included
  • IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL2 antibody included
  • IF Staining of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

    IF Staining of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUL2 antibody (67175-1-Ig, Clone: 1A6G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CUL2 Monoclonal antibody, PBS Only

CUL2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67175-1-PBS

产品说明书

CloneNo. 1A6G9

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Cullin-2, cullin 2, CUL-2, CUL 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CUL2 Ready-To-Use IHC Kit. CUL2 primary antibody included.

产品信息

67175-1-PBS targets CUL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 CUL2 fusion protein Ag28654 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cullin 2
别名 Cullin-2, cullin 2, CUL-2, CUL 2
计算分子量 87 kDa
观测分子量 85-90 kDa
GenBank蛋白编号BC009591
基因名称 CUL2
Gene ID (NCBI) 8453
RRIDAB_2882471
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13617
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.