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CUL2 Monoclonal antibody

CUL2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1A6G9

Cat no : 67175-1-Ig

Print datasheet

Synonyms

CUL 2, CUL2, cullin 2

验证数据展示

  • WB analysis of MCF-7 using 67175-1-Ig

    WB analysis of MCF-7 using 67175-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 67175-1-Ig

    WB analysis of Raji using 67175-1-Ig

    WB result of CUL2 antibody (67175-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected Raji cells.

  • IHC staining of human breast cancer using 67175-1-Ig

    IHC staining of human breast cancer using 67175-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67175-1-Ig

    IHC staining of human breast cancer using 67175-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67175-1-Ig

    IF Staining of HeLa using 67175-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUL2 antibody (67175-1-Ig, Clone: 1A6G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, PC-3 cells, HSC-T6 cells, Raji cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67175-1-Ig targets CUL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity Human, Rat
Cited Reactivityhuman
Immunogen CUL2 fusion protein Ag28654 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cullin 2
Synonyms CUL 2, CUL2, cullin 2
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberBC009591
Gene Symbol CUL2
Gene ID (NCBI) 8453
RRIDAB_2882471
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13617
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

实验方案

Product Specific Protocols
WB protocol for CUL2 antibody 67175-1-IgDownload protocol
IHC protocol for CUL2 antibody 67175-1-IgDownload protocol
IF protocol for CUL2 antibody 67175-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,IF

FEBS J

TRAF2 associates with cullin neddylation complex assembly

Authors - Tiantian Wang
View Article