验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CUL2 Ready-To-Use IHC Kit. CUL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67175-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67175 targets CUL2 in IF/ICC applications and shows reactivity with Human, Rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Rat |
| 免疫原 | CUL2 fusion protein Ag28654 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cullin 2 |
| 别名 | CUL 2, CUL2, cullin 2 |
| 计算分子量 | 87 kDa |
| 观测分子量 | 85-90 kDa |
| GenBank蛋白编号 | BC009591 |
| 基因名称 | CUL2 |
| Gene ID (NCBI) | 8453 |
| RRID | AB_3084321 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q13617 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CUL2 antibody CL488-67175 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |