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  • Featured Product
  • KD/KO Validated

CUL4A Monoclonal antibody

CUL4A Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, monkey, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1A7F12

Cat no : 66038-1-Ig

Print datasheet

Synonyms

CUL 4A, CUL4A, cullin 4A

验证数据展示

  • WB analysis using 66038-1-Ig

    WB analysis using 66038-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of MCF-7 using 66038-1-Ig

    IP experiment of MCF-7 using 66038-1-Ig

    IP result of anti-CUL4A (IP:66038-1-Ig, 4ug; Detection:66038-1-Ig 1:500) with MCF-7 cells lysate 2800ug.

  • IHC staining of human heart using 66038-1-Ig

    IHC staining of human heart using 66038-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with CUL4A antibody included
  • IHC staining of human heart using 66038-1-Ig

    IHC staining of human heart using 66038-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CUL4A antibody included
  • IF Staining of HepG2 using 66038-1-Ig

    IF Staining of HepG2 using 66038-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66038-1-Ig (CUL4A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, pig brain tissue, MCF-7 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman heart tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CUL4A Ready-To-Use IHC Kit. CUL4A primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66038-1-Ig targets CUL4A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, monkey, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, monkey, mouse, rat, pig
Cited Reactivityhuman, mouse
Immunogen CUL4A fusion protein Ag18089 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cullin 4A
Synonyms CUL 4A, CUL4A, cullin 4A
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 77 kDa, 88 kDa
GenBank Accession NumberBC008308
Gene Symbol CUL4A
Gene ID (NCBI) 8451
RRIDAB_11042446
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13619
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

实验方案

Product Specific Protocols
WB protocol for CUL4A antibody 66038-1-IgDownload protocol
IHC protocol for CUL4A antibody 66038-1-IgDownload protocol
IF protocol for CUL4A antibody 66038-1-IgDownload protocol
IP protocol for CUL4A antibody 66038-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Curr Biol

Temporal Regulation of ESCO2 Degradation by the MCM Complex, the CUL4-DDB1-VPRBP Complex, and the Anaphase-Promoting Complex.

Authors - Masashi Minamino
View Article
humanWB

Oncogene

GSK3β-driven SOX2 overexpression is a targetable vulnerability in esophageal squamous cell carcinoma

Authors - Li Kang
  • KD Validated
View Article
mouseWB,IF,IP

Stem Cell Res Ther

KAP1 phosphorylation promotes the survival of neural stem cells after ischemia/reperfusion by maintaining the stability of PCNA.

Authors - Wan Wang
View Article
humanWB,IP

Cell Death Differ

AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner

Authors - Weimin Xu
View Article