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  • Featured Product
  • KD/KO Validated

CUL4A Monoclonal antibody, PBS Only

CUL4A Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, monkey, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A7F12

Cat no : 66038-1-PBS

Print datasheet

Synonyms

CUL 4A, CUL4A, cullin 4A

验证数据展示

  • WB analysis using 66038-1-Ig (same clone as 66038-1-PBS)

    WB analysis using 66038-1-Ig (same clone as 66038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 66038-1-Ig (same clone as 66038-1-PBS)

    IP experiment of MCF-7 using 66038-1-Ig (same clone as 66038-1-PBS)

    IP result of anti-CUL4A (IP:66038-1-Ig, 4ug; Detection:66038-1-Ig 1:500) with MCF-7 cells lysate 2800ug. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66038-1-Ig (same clone as 66038-1-PBS)

    IF Staining of HepG2 using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66038-1-Ig (CUL4A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

产品信息

66038-1-PBS targets CUL4A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, monkey, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, monkey, mouse, rat, pig
Immunogen CUL4A fusion protein Ag18089 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cullin 4A
Synonyms CUL 4A, CUL4A, cullin 4A
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 77 kDa, 88 kDa
GenBank Accession NumberBC008308
Gene Symbol CUL4A
Gene ID (NCBI) 8451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13619
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.