验证数据展示
产品信息
66038-1-PBS targets CUL4A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, monkey, mouse, rat, pig samples.
经测试应用 | WB, IHC, IF/ICC, IP, Indirect ELISA Application Description |
经测试反应性 | human, monkey, mouse, rat, pig |
免疫原 | CUL4A fusion protein Ag18089 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | cullin 4A |
别名 | CUL 4A, CUL4A, cullin 4A |
计算分子量 | 77 kDa |
观测分子量 | 77 kDa, 88 kDa |
GenBank蛋白编号 | BC008308 |
基因名称 | CUL4A |
Gene ID (NCBI) | 8451 |
RRID | AB_11042446 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q13619 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.