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CUL4B Monoclonal antibody

CUL4B Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60151-1-Ig

产品说明书

CloneNo. 4E10B10

宿主/亚型

Mouse / IgG1

种属反应性

human, rat and More (1)

应用

WB, IHC, ELISA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, ROS1728 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60151-1-Ig targets CUL4B in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, rat
文献引用反应性human, mouse
免疫原 CUL4B fusion protein Ag3563 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cullin 4B
别名
计算分子量 913 aa, 104 kDa
观测分子量 102 kDa
GenBank蛋白编号BC036216
基因名称 CUL4B
Gene ID (NCBI) 8450
RRIDAB_10641034
偶联类型 Unconjugated
形式 Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDQ13620
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

实验方案

Product Specific Protocols
WB protocol for CUL4B antibody 60151-1-IgDownload protocol
IHC protocol for CUL4B antibody 60151-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Curr Biol

Temporal Regulation of ESCO2 Degradation by the MCM Complex, the CUL4-DDB1-VPRBP Complex, and the Anaphase-Promoting Complex.

Authors - Masashi Minamino

Int J Oncol

miR‑381 and miR‑489 suppress cell proliferation and invasion by targeting CUL4B via the Wnt/β‑catenin pathway in gastric cancer.

Authors - Ziling Fang
mouseWB

Biochem Biophys Res Commun

Insulin alleviates LPS-induced ARDS via inhibiting CUL4B-mediated proteasomal degradation and restoring expression level of Na,K-ATPase α1 subunit through elevating HCF-1.

Authors - Xue-Ting Huang
humanIHC, WB

Pathol Res Pract

Cullin-4B promotes cell proliferation and invasion through inactivation of p53 signaling pathway in colorectal cancer.

Authors - Min Zhong
humanWB

Sci Adv

NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors

Authors - Alice Meroni
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