• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60151-1-Ig (same clone as 60151-1-PBS)

    WB analysis using 60151-1-Ig (same clone as 60151-1-PBS)

    various lysates were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa cells using 60151-1-Ig (same clone as 60151-1-PBS)

    WB analysis of HeLa cells using 60151-1-Ig (same clone as 60151-1-PBS)

    WB result of CUL4B antibody (60151-1-Ig, 1:500) with si-Control and si-CUL4B transfected HeLa cells. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60151-1-Ig (same clone as 60151-1-PBS)

    WB analysis of HeLa using 60151-1-Ig (same clone as 60151-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL4B antibody included
  • IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL4B antibody included
  • Featured Product
  • KD/KO验证

CUL4B Monoclonal antibody, PBS Only

CUL4B Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60151-1-PBS

产品说明书

CloneNo. 4E10B10

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.

产品信息

60151-1-PBS targets CUL4B in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 CUL4B fusion protein Ag3563 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cullin 4B
别名
计算分子量 913 aa, 104 kDa
观测分子量 102 kDa
GenBank蛋白编号BC036216
基因名称 CUL4B
Gene ID (NCBI) 8450
RRIDAB_10641034
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13620
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).