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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60151-1-Ig (same clone as 60151-1-PBS)

    WB analysis using 60151-1-Ig (same clone as 60151-1-PBS)

    various lysates were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa cells using 60151-1-Ig (same clone as 60151-1-PBS)

    WB analysis of HeLa cells using 60151-1-Ig (same clone as 60151-1-PBS)

    WB result of CUL4B antibody (60151-1-Ig, 1:500) with si-Control and si-CUL4B transfected HeLa cells. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60151-1-Ig (same clone as 60151-1-PBS)

    WB analysis of HeLa using 60151-1-Ig (same clone as 60151-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL4B antibody included
  • IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL4B antibody included
  • Featured Product
  • KD/KO Validated

CUL4B Monoclonal antibody, PBS Only

CUL4B Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60151-1-PBS

Print datasheet

CloneNo. 4E10B10

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.

产品信息

60151-1-PBS targets CUL4B in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen CUL4B fusion protein Ag3563 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cullin 4B
Synonyms
Calculated Molecular Weight 913 aa, 104 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC036216
Gene Symbol CUL4B
Gene ID (NCBI) 8450
RRIDAB_10641034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13620
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).