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验证数据展示

  • IF Staining of HeLa using 85001-4-RR

    IF Staining of HeLa using 85001-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (85001-4-RR, Clone: 242626F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 85001-4-RR

    FC experiment of HeLa using 85001-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CUL4B Recombinant antibody (85001-4-RR, Clone:242626F11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85001-4-RR

    Affinity and Kinetic Characterization of 85001-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85001-4-RR against Human CUL4B were performed. The affinity constant is 0.51 nM.

CUL4B Recombinant antibody

CUL4B Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85001-4-RR

产品说明书

CloneNo. 242626F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

242626F11, cullin 4B, Cullin-4B, KIAA0695

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85001-4-RR targets CUL4B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 CUL4B fusion protein Ag3563 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cullin 4B
别名 242626F11, cullin 4B, Cullin-4B, KIAA0695
计算分子量 913 aa, 104 kDa
GenBank蛋白编号BC036216
基因名称 CUL4B
Gene ID (NCBI) 8450
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13620
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

实验方案

Product Specific Protocols
IF protocol for CUL4B antibody 85001-4-RRDownload protocol
FC protocol for CUL4B antibody 85001-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols