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验证数据展示

  • IF Staining of HeLa using 85001-4-RR

    IF Staining of HeLa using 85001-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (85001-4-RR, Clone: 242626F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85001-4-RR

    Affinity and Kinetic Characterization of 85001-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85001-4-RR against Human CUL4B were performed. The affinity constant is 0.51 nM.

CUL4B Recombinant antibody

CUL4B Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 85001-4-RR

Print datasheet

CloneNo. 242626F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

KIAA0695, Cullin-4B, cullin 4B, 242626F11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85001-4-RR targets CUL4B in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CUL4B fusion protein Ag3563 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cullin 4B
Synonyms KIAA0695, Cullin-4B, cullin 4B, 242626F11
Calculated Molecular Weight 913 aa, 104 kDa
GenBank Accession NumberBC036216
Gene Symbol CUL4B
Gene ID (NCBI) 8450
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13620
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

实验方案

Product Specific Protocols
IF protocol for CUL4B antibody 85001-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols