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  • Featured Product
  • KD/KO Validated

CUL7 Monoclonal antibody, PBS Only

CUL7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E3G9

Cat no : 67034-1-PBS

Print datasheet

Synonyms

CUL 7, CUL7, cullin 7, dJ20C7.5, KIAA0076

验证数据展示

  • WB analysis of HEK-293 using 67034-1-Ig (same clone as 67034-1-PBS)

    WB analysis of HEK-293 using 67034-1-Ig (same clone as 67034-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 67034-1-Ig (same clone as 67034-1-PBS)

    IHC staining of human heart using 67034-1-Ig (same clone as 67034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67034-1-Ig (CUL7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 67034-1-Ig (same clone as 67034-1-PBS)

    IHC staining of human heart using 67034-1-Ig (same clone as 67034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67034-1-Ig (CUL7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67034-1-Ig (same clone as 67034-1-PBS)

    WB analysis of HEK-293 using 67034-1-Ig (same clone as 67034-1-PBS)

    WB result of CUL7 antibody (67034-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL7 transfected HEK-293 cells. This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

产品信息

67034-1-PBS targets CUL7 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CUL7 fusion protein Ag6943 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cullin 7
Synonyms CUL 7, CUL7, cullin 7, dJ20C7.5, KIAA0076
Calculated Molecular Weight 1698 aa, 191 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC033647
Gene Symbol CUL7
Gene ID (NCBI) 9820
RRIDAB_2882349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14999
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

he cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. CUL7 was originally discovered as 185-kDa protein associated with the large T antigen of simian virus 40 (SV40). CUL7-deficient mice exhibit neonatal lethality with reduced size and vascular defects. CUL7 presumably plays a role in the DNA damage response by limiting p53 activity. CUL7 mutations have also been identified in 3-Msyndrome and the Yakuts short stature syndrome, both of which are characterized by pre- and post-natal growth retardation but with relatively normal mental and endocrine functions, suggesting that CUL7 may also be crucial for human placental development.