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  • Featured Product
  • KD/KO Validated

CUX2 Recombinant antibody

CUX2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230235G1

Cat no : 82933-1-RR

Print datasheet

Synonyms

Homeobox protein cux-2, Homeobox protein cut-like 2, CUTL2, cut like homeobox 2, CDP2

验证数据展示

  • WB analysis using 82933-1-RR

    WB analysis using 82933-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82933-1-RR (CUX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 82933-1-RR

    WB analysis of SH-SY5Y using 82933-1-RR

    WB result of CUX2 antibody (82933-1-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUX2 transfected SH-SY5Y cells.

  • IF Staining of HeLa using 82933-1-RR

    IF Staining of HeLa using 82933-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 82933-1-RR

    IF Staining of HeLa using 82933-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82933-1-RR

    Affinity and Kinetic Characterization of 82933-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82933-1-RR against Human CUX2 were performed. The affinity constant is 1.02 nM.

经过测试的应用

Positive WB detected inK-562 cells, SH-SY5Y cells, HepG2 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82933-1-RR targets CUX2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CUX2 fusion protein Ag18809 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cut-like homeobox 2
Synonyms Homeobox protein cux-2, Homeobox protein cut-like 2, CUTL2, cut like homeobox 2, CDP2
Calculated Molecular Weight 1486 aa, 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberBC151245
Gene Symbol CUX2
Gene ID (NCBI) 23316
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14529
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cut like homeobox 2 (CUX2) also named as, CDP2 or CUTL2, is a 1486 amino acid protein, which contains one homeobox DNA-binding domain and three CUT DNA-binding domains. CUX2 localizes in the nucleus and belongs to the CUT homeobox family. CUX2 may as a transcription factor be involved in neural specification.

实验方案

Product Specific Protocols
WB protocol for CUX2 antibody 82933-1-RRDownload protocol
IF protocol for CUX2 antibody 82933-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols