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Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody

CXCL8/IL-8 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240889B2

Cat no : 98137-1-RR

Print datasheet

Synonyms

CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif

验证数据展示

  • FC experiment of human PBMCs using 98137-1-RR

    FC experiment of human PBMCs using 98137-1-RR

    1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Granulocytes were gated.

  • FC experiment of human PBMCs using 98137-1-RR

    FC experiment of human PBMCs using 98137-1-RR

    1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Granulocytes were gated.

  • Affinity and Kinetic Characterization of 98137-1-RR

    Affinity and Kinetic Characterization of 98137-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98137-1-RR against Human CXCL8/IL-8 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive FC (Intra) detected inIFN gamma, LPS and Brefeldin A treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy CXCL8/IL-8 Ready-To-Use IHC Kit. CXCL8/IL-8 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98137-1-RR is CXCL8/IL-8 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 8
Synonyms CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif
Calculated Molecular Weight 99 aa, 11 kDa
GenBank Accession NumberBC013615
Gene Symbol IL-8
Gene ID (NCBI) 3576
ENSEMBL Gene IDENSG00000169429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10145
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interleukin 8 (IL-8), also known as CXCL8, is a CXC chemokine family member. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

实验方案

Product Specific Protocols
FC protocol for CXCL8/IL-8 antibody 98137-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols