验证数据展示
经过测试的应用
Positive FC (Intra) detected in | IFN gamma, LPS and Brefeldin A treated human PBMCs |
推荐稀释比
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
98137-1-RR targets CXCL8/IL-8 in FC (Intra) applications and shows reactivity with human samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | Recombinant protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | interleukin 8 |
Synonyms | CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif |
Calculated Molecular Weight | 99 aa, 11 kDa |
GenBank Accession Number | BC013615 |
Gene Symbol | IL-8 |
Gene ID (NCBI) | 3576 |
ENSEMBL Gene ID | ENSG00000169429 |
RRID | AB_3672283 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P10145 |
Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
Interleukin 8 (IL-8), also known as CXCL8, is a CXC chemokine family member. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.
实验方案
Product Specific Protocols | |
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FC protocol for CXCL8/IL-8 antibody 98137-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |