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验证数据展示

  • FC experiment of human PBMCs using 98137-1-RR

    FC experiment of human PBMCs using 98137-1-RR

    1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human PBMCs using 98137-1-RR

    FC experiment of human PBMCs using 98137-1-RR

    1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98137-1-RR

    Affinity and Kinetic Characterization of 98137-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98137-1-RR against Human CXCL8/IL-8 were performed. The affinity constant is below 1 pM.

Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody

CXCL8/IL-8 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98137-1-RR

产品说明书

CloneNo. 240889B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inIFN gamma, LPS and Brefeldin A treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy CXCL8/IL-8 Ready-To-Use IHC Kit. CXCL8/IL-8 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98137-1-RR targets CXCL8/IL-8 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 8
别名 CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif
计算分子量 99 aa, 11 kDa
GenBank蛋白编号BC013615
基因名称 IL-8
Gene ID (NCBI) 3576
ENSEMBL Gene IDENSG00000169429
RRIDAB_3672283
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10145
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interleukin 8 (IL-8), also known as CXCL8, is a CXC chemokine family member. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

实验方案

Product Specific Protocols
FC protocol for CXCL8/IL-8 antibody 98137-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols