• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using 98211-1-RR

    FC experiment of human PBMCs using 98211-1-RR

    1x10^6 untreated or IFN gamma, TNF α and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL9/MIG Rabbit Recombinant Antibody (98211-1-RR, Clone: 241437F5). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human PBMCs using 98211-1-RR

    FC experiment of human PBMCs using 98211-1-RR

    1x10^6 IFN gamma, TNF α and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL9/MIG Rabbit Recombinant Antibody (98211-1-RR, Clone: 241437F5) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL9/MIG Rabbit Recombinant Antibody (98211-1-RR, Clone: 241437F5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Anti-Human CXCL9/MIG Rabbit Recombinant Antibody

CXCL9/MIG Recombinant Antibody for FC (Intra)
Cat No. 98211-1-RR

产品说明书

CloneNo. 241437F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

MIG; CXCL9, MIG, CXCL9, C-X-C motif chemokine 9, CMK

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC (Intra) detected inIFN gamma, TNF α and Brefeldin A treated human PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98211-1-RR targets CXCL9/MIG in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 CXCL9/MIG fusion protein Ag17932 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X-C motif) ligand 9
别名 MIG; CXCL9, MIG, CXCL9, C-X-C motif chemokine 9, CMK
计算分子量 14 kDa
GenBank蛋白编号BC095396
基因名称 CXCL9
Gene ID (NCBI) 4283
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ07325
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CXCL9 is a small cytokine known as MIG. CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. A high level of CXCL9 revealed in peripheral liquids, can be considered as a marker of host immune response, especially of that involving Th1 cells.

实验方案

Product Specific Protocols
FC protocol for CXCL9/MIG antibody 98211-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols