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Anti-Human CXCL9/MIG Rabbit Recombinant Antibody

CXCL9/MIG Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241437F5

Cat no : 98211-1-RR

Print datasheet

Synonyms

MIG; CXCL9, MIG, CXCL9, C-X-C motif chemokine 9, CMK

验证数据展示

  • FC experiment of human PBMCs using 98211-1-RR

    FC experiment of human PBMCs using 98211-1-RR

    1x10^6 untreated or IFN gamma, TNF α and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL9/MIG Rabbit Recombinant Antibody (98211-1-RR, Clone: 241437F5). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human PBMCs using 98211-1-RR

    FC experiment of human PBMCs using 98211-1-RR

    1x10^6 IFN gamma, TNF α and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL9/MIG Rabbit Recombinant Antibody (98211-1-RR, Clone: 241437F5) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL9/MIG Rabbit Recombinant Antibody (98211-1-RR, Clone: 241437F5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inIFN gamma, TNF α and Brefeldin A treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98211-1-RR targets CXCL9/MIG in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen CXCL9/MIG fusion protein Ag17932 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-X-C motif) ligand 9
Synonyms MIG; CXCL9, MIG, CXCL9, C-X-C motif chemokine 9, CMK
Calculated Molecular Weight 14 kDa
GenBank Accession NumberBC095396
Gene Symbol CXCL9
Gene ID (NCBI) 4283
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ07325
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CXCL9 is a small cytokine known as MIG. CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. A high level of CXCL9 revealed in peripheral liquids, can be considered as a marker of host immune response, especially of that involving Th1 cells.

实验方案

Product Specific Protocols
FC protocol for CXCL9/MIG antibody 98211-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols