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验证数据展示

  • WB analysis using 84639-2-RR

    WB analysis using 84639-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84639-2-RR (CXCR5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CXCR5 Recombinant antibody

CXCR5 Recombinant Antibody for WB, ELISA
Cat No. 84639-2-RR
CloneNo. 241777A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CXCR 5, CXC R5, CD185, Burkitt lymphoma receptor 1, BLR1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells, Jurkat cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84639-2-RR targets CXCR5 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-X-C motif) receptor 5
Synonyms CXCR 5, CXC R5, CD185, Burkitt lymphoma receptor 1, BLR1
Calculated Molecular Weight 372 aa, 42 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC110352
Gene Symbol CXCR5
Gene ID (NCBI) 643
RRIDAB_3672098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32302
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Initially discovered in 1992 as Burkitt's lymphoma receptor 1, CXCR5 (chemokine receptor 5, also known as CD185) is a seven-transmembrane G protein-coupled receptor (GPCR) protein (PMID: 32994482). CXCR5 is essential for naïve B-cell migration to and maturation in the lymph nodes and spleen, as well as for cluster of differentiation 4 T-cell (TFH and TCM) homing and interaction with B cells (PMID: 21471443). Moreover, CXCR5 is involved in the regulation of neural stem cells (NSC) and neuronal regeneration in the mouse brain, neuronal differentiation, neurogenesis, gliogenesis, and synaptogenesis (PMID: 37460877).

实验方案

Product Specific Protocols
WB protocol for CXCR5 antibody 84639-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols