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CXorf21 Polyclonal antibody

CXorf21 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30244-1-AP

Print datasheet

Synonyms

CXorf21, FLJ11577

验证数据展示

  • WB analysis using 30244-1-AP

    WB analysis using 30244-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30244-1-AP (CXorf21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 30244-1-AP

    IF Staining of U2OS using 30244-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CXorf21 antibody (30244-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U-251 using 30244-1-AP

    IF Staining of U-251 using 30244-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CXorf21 antibody (30244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inTHP-1 cells
Positive IF/ICC detected inU-251 cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy TASL/CXorf21 Ready-To-Use IHC Kit. TASL/CXorf21 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30244-1-AP targets CXorf21 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen CXorf21 fusion protein Ag32773 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome X open reading frame 21
Synonyms CXorf21, FLJ11577
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC020611
Gene Symbol CXorf21
Gene ID (NCBI) 80231
RRIDAB_3086276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXorf21 (Chromosome X open reading frame 21) is also named as TASL and TLR adapter interacting with SLC15A4 on the lysosome. CXorf21 is an innate immune adapter that mediates the recruitment and activation of IRF5 downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PMID:32433612). CXorf21 plays a role in the regulation of endolysosomal pH in immune cells such as B-cells, dendritic cells and monocytes (PMID: 31001245). CXorf21 is a mediator of the X-chromosome gene dose-dependent increased risk of SLE in females (PMID: 31695690).

实验方案

Product Specific Protocols
WB protocol for CXorf21 antibody 30244-1-APDownload protocol
IF protocol for CXorf21 antibody 30244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols