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验证数据展示

  • WB analysis using 30244-1-AP

    WB analysis using 30244-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30244-1-AP (CXorf21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 30244-1-AP

    IHC staining of mouse skin using 30244-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TASL/CXorf21 antibody included
  • IHC staining of mouse skin using 30244-1-AP

    IHC staining of mouse skin using 30244-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TASL/CXorf21 antibody included
  • IF Staining of U2OS using 30244-1-AP

    IF Staining of U2OS using 30244-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CXorf21 antibody (30244-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human cervical cancer using 30244-1-AP

    IHC staining of human cervical cancer using 30244-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TASL/CXorf21 antibody included
  • IHC staining of rat skin using 30244-1-AP

    IHC staining of rat skin using 30244-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TASL/CXorf21 antibody included
  • IHC staining of rat skin using 30244-1-AP

    IHC staining of rat skin using 30244-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TASL/CXorf21 antibody included
  • IHC staining of rat stomach using 30244-1-AP

    IHC staining of rat stomach using 30244-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TASL/CXorf21 antibody included
  • IF Staining of U-251 using 30244-1-AP

    IF Staining of U-251 using 30244-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CXorf21 antibody (30244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

CXorf21 Polyclonal antibody

CXorf21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30244-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

TASL, TLR adapter interacting with SLC15A4 on the lysosome

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells
Positive IHC detected inmouse skin tissue, human cervical cancer tissue, rat skin tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy TASL/CXorf21 Ready-To-Use IHC Kit. TASL/CXorf21 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30244-1-AP targets CXorf21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CXorf21 fusion protein Ag32773 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome X open reading frame 21
别名 TASL, TLR adapter interacting with SLC15A4 on the lysosome
观测分子量 34 kDa
GenBank蛋白编号BC020611
基因名称 CXorf21
Gene ID (NCBI) 80231
RRIDAB_3086276
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXorf21 (Chromosome X open reading frame 21) is also named as TASL and TLR adapter interacting with SLC15A4 on the lysosome. CXorf21 is an innate immune adapter that mediates the recruitment and activation of IRF5 downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PMID:32433612). CXorf21 plays a role in the regulation of endolysosomal pH in immune cells such as B-cells, dendritic cells and monocytes (PMID: 31001245). CXorf21 is a mediator of the X-chromosome gene dose-dependent increased risk of SLE in females (PMID: 31695690).

实验方案

Product Specific Protocols
WB protocol for CXorf21 antibody 30244-1-APDownload protocol
IHC protocol for CXorf21 antibody 30244-1-APDownload protocol
IF protocol for CXorf21 antibody 30244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols