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验证数据展示

  • WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    WB results of CYB5R3 antibody (66022-1-Ig, 1:60000) with si-Control and si-CYB5R3 transfected HeLa cells. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66022-1-Ig (same clone as 66022-1-PBS)

    IHC staining of human liver cancer using 66022-1-Ig (same clone as 66022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IHC staining of human liver cancer using 66022-1-Ig (same clone as 66022-1-PBS)

    IHC staining of human liver cancer using 66022-1-Ig (same clone as 66022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IF Staining of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    IF Staining of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    Western blot analysis of CYB5R3-specific in various tissues using Proteintech antibody 66022-1-Ig at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CYB5R3 Monoclonal antibody, PBS Only

CYB5R3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66022-1-PBS

产品说明书

CloneNo. 9C4D10

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CYB5R3 Ready-To-Use IHC Kit. CYB5R3 primary antibody included.

产品信息

66022-1-PBS targets CYB5R3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 CYB5R3 fusion protein Ag18096 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cytochrome b5 reductase 3
别名 B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC004821
基因名称 CYB5R3
Gene ID (NCBI) 1727
RRIDAB_11045662
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00387
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.