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  • Featured Product
  • KD/KO Validated

CYB5R3 Monoclonal antibody, PBS Only

CYB5R3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

9C4D10

Cat no : 66022-1-PBS

Print datasheet

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

验证数据展示

  • WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    WB results of CYB5R3 antibody (66022-1-Ig, 1:60000) with si-Control and si-CYB5R3 transfected HeLa cells. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66022-1-Ig (same clone as 66022-1-PBS)

    IHC staining of human liver cancer using 66022-1-Ig (same clone as 66022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IHC staining of human liver cancer using 66022-1-Ig (same clone as 66022-1-PBS)

    IHC staining of human liver cancer using 66022-1-Ig (same clone as 66022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IF Staining of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    IF Staining of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    WB analysis of HeLa using 66022-1-Ig (same clone as 66022-1-PBS)

    Western blot analysis of CYB5R3-specific in various tissues using Proteintech antibody 66022-1-Ig at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66022-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CYB5R3 Ready-To-Use IHC Kit. CYB5R3 primary antibody included.

产品信息

66022-1-PBS targets CYB5R3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CYB5R3 fusion protein Ag18096 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cytochrome b5 reductase 3
Synonyms B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC004821
Gene Symbol CYB5R3
Gene ID (NCBI) 1727
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00387
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.